Please use this identifier to cite or link to this item: https://hdl.handle.net/20.500.11851/10344
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dc.contributor.authorAlangari, Mashari-
dc.contributor.authorDemir, Büsra-
dc.contributor.authorAkin Gültaktı, Çağlanaz-
dc.contributor.authorÖren, Ersin Emre-
dc.contributor.authorHihath, Joshua-
dc.date.accessioned2023-04-16T10:01:15Z-
dc.date.available2023-04-16T10:01:15Z-
dc.date.issued2023-
dc.identifier.citationAlangari, M., Demir, B., Gultakti, C. A., Oren, E. E., & Hihath, J. (2023). Mapping DNA Conformations Using Single-Molecule Conductance Measurements. Biomolecules, 13(1), 129.en_US
dc.identifier.issn2218-273X-
dc.identifier.urihttps://doi.org/10.3390/biom13010129-
dc.identifier.urihttps://hdl.handle.net/20.500.11851/10344-
dc.description.abstractDNA is an attractive material for a range of applications in nanoscience and nanotechnology, and it has recently been demonstrated that the electronic properties of DNA are uniquely sensitive to its sequence and structure, opening new opportunities for the development of electronic DNA biosensors. In this report, we examine the origin of multiple conductance peaks that can occur during single-molecule break-junction (SMBJ)-based conductance measurements on DNA. We demonstrate that these peaks originate from the presence of multiple DNA conformations within the solutions, in particular, double-stranded B-form DNA (dsDNA) and G-quadruplex structures. Using a combination of circular dichroism (CD) spectroscopy, computational approaches, sequence and environmental controls, and single-molecule conductance measurements, we disentangle the conductance information and demonstrate that specific conductance values come from specific conformations of the DNA and that the occurrence of these peaks can be controlled by controlling the local environment. In addition, we demonstrate that conductance measurements are uniquely sensitive to identifying these conformations in solutions and that multiple configurations can be detected in solutions over an extremely large concentration range, opening new possibilities for examining low-probability DNA conformations in solutions.en_US
dc.description.sponsorshipNational Science Foundation [NSF-1807555/2036865]en_US
dc.description.sponsorshipThis research was funded by National Science Foundation NSF-1807555/2036865 and from Quantum Biosystems Inc.en_US
dc.language.isoenen_US
dc.publisherMDPIen_US
dc.relation.ispartofBiomoleculesen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectsingle-molecule electronicsen_US
dc.subjectmolecular electronicsen_US
dc.subjectsingle-molecule break junctionen_US
dc.subjectDNAen_US
dc.subjectG-quadruplexesen_US
dc.subjectCircular-Dichroismen_US
dc.subjectG-Quadruplexesen_US
dc.subjectQuartet Structuresen_US
dc.subjectNucleic-Acidsen_US
dc.subjectCd Spectraen_US
dc.subjectIdentificationen_US
dc.subjectWateren_US
dc.subjectLongen_US
dc.titleMapping Dna Conformations Using Single-Molecule Conductance Measurementsen_US
dc.typeArticleen_US
dc.departmentTOBB ETÜen_US
dc.departmentFaculties, Faculty of Engineering, Department of Biomedical Engineeringen_US
dc.departmentFakülteler, Mühendislik Fakültesi, Biyomedikal Mühendisliği Bölümütr_TR
dc.identifier.volume13en_US
dc.identifier.issue1en_US
dc.authoridDemir, Busra/0000-0002-3911-2291-
dc.authoridHihath, Joshua/0000-0002-2949-9293-
dc.identifier.wosWOS:000914975800001en_US
dc.identifier.scopus2-s2.0-85146801220en_US
dc.institutionauthor-
dc.identifier.pmid36671514en_US
dc.identifier.doi10.3390/biom13010129-
dc.authorwosidDemir, Busra/W-1919-2018-
dc.authorscopusid57204556287-
dc.authorscopusid57204554850-
dc.authorscopusid58075821500-
dc.authorscopusid35846321000-
dc.authorscopusid9436467600-
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.identifier.scopusqualityQ1-
item.openairetypeArticle-
item.languageiso639-1en-
item.grantfulltextopen-
item.fulltextWith Fulltext-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.cerifentitytypePublications-
crisitem.author.dept02.2. Department of Biomedical Engineering-
Appears in Collections:PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection
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