Please use this identifier to cite or link to this item: https://hdl.handle.net/20.500.11851/8934
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dc.contributor.authorEren, Selen Kucukkaya-
dc.contributor.authorZirh, Elham Bahador-
dc.contributor.authorZirh, Selim-
dc.contributor.authorSharafi, Parisa-
dc.contributor.authorZeybek, Naciye Dilara-
dc.date.accessioned2022-11-30T19:23:57Z-
dc.date.available2022-11-30T19:23:57Z-
dc.date.issued2022-
dc.identifier.issn1678-7757-
dc.identifier.issn1678-7765-
dc.identifier.urihttps://doi.org/10.1590/1678-7757-2022-0086-
dc.identifier.urihttps://hdl.handle.net/20.500.11851/8934-
dc.description.abstractBioactive molecules present the potential to be used along with biomaterials in vital pulp therapy and regenerative endodontic treatment. Objective: The aim of this study was to assess the effects of the combined use of bone morphogenetic protein-7 (BMP-7) and mineral trioxide aggregate (MTA) on the proliferation, migration, and differentiation of human dental pulp stem cells (DPSCs). Methodology: For the proliferation analysis, DPSCs were incubated with a growth medium and treated with MTA and/or BMP-7 at different concentrations. For the following analyses, DPSCs were incubated with a differentiation medium and treated with MTA and/ or BMP-7. Moreover, there were groups in which DPSCs were incubated with the growth medium (control), the differentiation medium, or DMEM/F12 containing fetal bovine serum, and not treated with MTA or BMP-7. Cell proliferation was analyzed using the WST-1 assay. The odontogenic/osteogenic differentiation was evaluated by immunocytochemistry, alkaline phosphatase (ALP) activity assay, alizarin red staining, and reverse transcription-quantitative polymerase chain reaction (RT-qPCR). Cell migration was evaluated using a wound-healing assay. Data were analyzed using analysis of variance and Tukey test (p=0.05). Results: The use of BMP-7 with MTA presented no significant effect on cell proliferation in comparison with the treatment with MTA alone (p>0.05), but showed higher ALP activity, increased mineralization, and higher expression of DMP1 and DSPP when compared with other groups (p<0.05). Nestin expression was higher in the control group than in groups treated with MTA and/or BMP-7 (p<0.05). The cell migration rate increased after treatment with MTA when compared with other groups in all periods of time (p<0.05). At 72 hours, the wound area was smaller in groups treated with MTA and/ or BMP-7 than in the control group (p<0.05). Conclusion: The use of BMP-7 with MTA increased odontogenic/osteogenic differentiation without adversely affecting proliferation and migration of DPSCs. The use of BMP-7 with MTA may improve treatment outcomes by increasing repair and regeneration capacity of DPSCs.en_US
dc.description.sponsorshipHacettepe University Scientific Research Projects Coordination Unit [THD-2020-18625]en_US
dc.description.sponsorshipThe authors declare no conflict of interest related to this study. This study was supported by the Hacettepe University Scientific Research Projects Coordination Unit (THD-2020-18625).en_US
dc.language.isoenen_US
dc.publisherUniv Sao Paulo Fac Odontologia Bauruen_US
dc.relation.ispartofJournal of Applied Oral Scienceen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectCalcium silicateen_US
dc.subjectCytotoxicityen_US
dc.subjectOsteogenic protein-1en_US
dc.subjectPulpotomyen_US
dc.subjectRegenerative endodonticsen_US
dc.subjectOdontogenic Differentiationen_US
dc.subjectIrreversible Pulpitisen_US
dc.subjectPartial Pulpotomyen_US
dc.subjectPermanent Molarsen_US
dc.subjectTeethen_US
dc.subjectTissueen_US
dc.subjectSialoproteinen_US
dc.subjectExpressionen_US
dc.subjectImmatureen_US
dc.subjectDeliveryen_US
dc.titleCombined Effects of Bone Morphogenetic Protein-7 and Mineral Trioxide Aggregate on the Proliferation, Migration, and Differentiation of Human Dental Pulp Stem Cellsen_US
dc.typeArticleen_US
dc.identifier.volume30en_US
dc.authoridBahador Zırh, Elham/0000-0002-6921-2365-
dc.authoridZIRH, Selim/0000-0002-7962-6078-
dc.identifier.wosWOS:000855941800001en_US
dc.identifier.scopus2-s2.0-85137775541en_US
dc.institutionauthorBahador Zirh, Elham-
dc.institutionauthorSharafi, Parisa-
dc.identifier.pmid36102412en_US
dc.identifier.doi10.1590/1678-7757-2022-0086-
dc.authorwosidBahador Zırh, Elham/GWV-3753-2022-
dc.authorwosidZIRH, Selim/AAP-4024-2020-
dc.authorscopusid57191403740-
dc.authorscopusid57221571018-
dc.authorscopusid57207246752-
dc.authorscopusid57193913814-
dc.authorscopusid8540689700-
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.identifier.scopusqualityQ1-
dc.ozel2022v3_Editen_US
item.openairetypeArticle-
item.languageiso639-1en-
item.grantfulltextnone-
item.fulltextNo Fulltext-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.cerifentitytypePublications-
crisitem.author.dept03.14. Department of Internal Medicine-
Appears in Collections:PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
Temel Tıp Bilimleri Bölümü / Department of Basic Medical Sciences
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection
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