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https://hdl.handle.net/20.500.11851/8964
Title: | Detection of Blastocystis Subtypes in Children With Functional Abdominal Pain and Celiac Disease in Çorum, Turkey | Authors: | Güreser A.S. Comba A. Karasartova D. Kosar N. Keskin A. Stensvold C.R. Taylan-Ozkan A. |
Keywords: | Blastocystis Celiac disease Children Functional abdominal pain Gastroenterology abdominal pain Adenoviridae adolescent adult Article Blastocystis celiac disease child controlled study demography diagnostic procedure DNA extraction DNA sequencing female Giardia human major clinical study male microbiological examination nucleotide sequence phylogenetic tree phylogeny polymerase chain reaction questionnaire Rotavirus Salmonella Sanger sequencing Shigella socioeconomics |
Publisher: | Tehran University of Medical Sciences | Abstract: | Background: Blastocystis has been associated with various symptoms of the gastrointestinal tract. We aimed to investigate the prevalence of Blastocystis in children with celiac disease (CeD) or functional abdominal pain (FAP) and to evaluate its subtypes (STs) with respect to demographic, socioeconomic and epidemiological factors. Methods: Overall, 161 fecal samples were collected from healthy children and patients with FAP or CeD in Hitit University Erol Olçok Research and Training Hospital, Corum, Turkey between 2016-2018. Samples were examined using both native-Lugol (NL) and trichrome-stained (TS) smears, and further analyses by PCR and Sanger sequencing were performed. A standard questionnaire was applied to obtain demographic, socioeconomic, epidemiological data. Results: Blastocystis was found in 10.6% of the total study population. Neither bacteria nor any other parasites were found, except for one Giardia (0.6%) in the CeD group. The presence/absence of the parasite was not found to be associated with demographic, socioeconomic and epidemiological factors. Blastocysis was detected in 11.5% (6/52) of the CeD, 7.7% (4/52) of the FAP, and 12.3% (7/57) of the healthy group. Diagnostic methods were similar in terms of Blastocystis detection (P= 0.671), and there was fair agreement between the NL, TS and PCR (Fleiss' Kappa=0.847, P=0.001). ST2 (42.8%) and ST3 (35.7%) were the predominant STs followed by ST1 (21.4%). Conclusion: We observed no difference between study groups in terms of Blastocystis prevalence. ST1, ST2 and ST3 subtypes were detected. Blastocystis prevalence and STs were not related to any of the demographic, socioeconomic and epidemiological factors. © 2022 Güreser et al. Published by Tehran University of Medical Sciences. | URI: | https://doi.org/10.18502/ijpa.v17i3.10619 https://hdl.handle.net/20.500.11851/8964 |
ISSN: | 1735-7020 |
Appears in Collections: | PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection Temel Tıp Bilimleri Bölümü / Department of Basic Medical Sciences WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection |
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