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Title: Engineered Chimeric Peptides as Antimicrobial Surface Coating Agents toward Infection-Free Implants
Authors: Yazıcı, Hilal
O'Neill, Mary B.
Kaçar, Turgay
Wilson, Brandon R.
Ören, Ersin Emre
Sarıkaya, Mehmet
Tamerler, Candan
Keywords: modular peptides and peptide-based implant coatings
surface functionalization
titanium binding peptides (tibp)
antimicrobial peptide (amp)
chimeric peptides
Issue Date: Mar-2016
Publisher: Amer Chemical Soc
Source: Yazici, H., O’Neill, M. B., Kacar, T., Wilson, B. R., Oren, E. E., Sarikaya, M., & Tamerler, C. (2016). Engineered chimeric peptides as antimicrobial surface coating agents toward infection-free implants. ACS applied materials & interfaces, 8(8), 5070-5081.
Abstract: Prevention of bacterial colonization and consequent biofilm formation remains a major challenge in implantable medical devices. Implant-associated infections are not only a major cause of implant failures but also their conventional treatment with antibiotics brings further complications due to the escalation in multidrug resistance to a variety of bacterial species. Owing to their unique properties, antimicrobial peptides (AMPs) have gained significant attention as effective agents to combat colonization of microorganisms. These peptides have been shown to exhibit a wide spectrum of activities with specificity to a target cell while having a low tendency for developing bacterial resistance. Engineering biomaterial surfaces that feature AMP properties, therefore, offer a promising approach to prevent implant infections. Here, we engineered a chimeric peptide with bifunctionality that both forms a robust solid-surface coating while presenting antimicrobial property. The individual domains of the chimeric peptides were evaluated for their solid-binding kinetics to titanium substrate as well as for their antimicrobial properties in solution. The antimicrobial efficacy of the chimeric peptide on the implant material was evaluated in vitro against infection by a variety of bacteria, including Streptococcus mutans, Staphylococcus. epidermidis, and Escherichia coli, which are commonly found in oral and orthopedic implant related surgeries. Our results demonstrate significant improvement in reducing bacterial colonization onto titanium surfaces below the detectable limit. Engineered chimeric peptides with freely displayed antimicrobial domains could be a potential solution for developing infection-free surfaces by engineering implant interfaces with highly reduced bacterial colonization property.
ISSN: 1944-8244
Appears in Collections:Biyomedikal Mühendisliği Bölümü / Department of Biomedical Engineering
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection

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