Please use this identifier to cite or link to this item: https://hdl.handle.net/20.500.11851/1029
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dc.contributor.authorKabay, Gözde-
dc.contributor.authorKaleli, Gizem-
dc.contributor.authorSultanova, Zahida-
dc.contributor.authorÖlmez, Tolga Tarkan-
dc.contributor.authorŞeker, Urartu Özgür Şafak-
dc.contributor.authorMutlu, Mehmet-
dc.date.accessioned2019-05-23T05:48:45Z
dc.date.available2019-05-23T05:48:45Z
dc.date.issued2016-06
dc.identifier.citationKabay, G., Kaleli, G., Sultanova, Z., Ölmez, T. T., Şeker, U. Ö. Ş., & Mutlu, M. (2016). Biocatalytic protein membranes fabricated by electrospinning. Reactive and Functional Polymers, 103, 26-32.en_US
dc.identifier.issn1381-5148
dc.identifier.othernumber of pages 7
dc.identifier.urihttps://doi.org/10.1016/j.reactfunctpolym.2016.03.015-
dc.identifier.urihttps://hdl.handle.net/20.500.11851/1029-
dc.description.abstractIn this study, a protein-based catalytic membrane was produced by electrospinning. Membrane activity was characterised in terms of response current for various glucose concentrations. We focused on the preparation of a scaffold by converting a globular protein to other structural forms using catastrophic solvents. A scaffolding protein, bovine serum albumin, and an enzyme, glucose oxidase (GOD), were selected as a model natural carrier matrix and a biologically active agent, respectively. Beta-mercaptoethanol (beta-ME) was used to convert the globular protein to an amyloid-like form. A structural stabilising agent, 2,2,2-triflouroethanol (TFE), was used to maintain the final alpha-helical structure of the amyloid-like protein. The TFE:PBS (phosphate-buffered saline) ratio and various electrospinning parameters were analysed to minimise activity loss. Using this approach, we applied electrospinning to an active enzyme to obtain biocatalytic nanofibrous membranes. After optimising the protein electrospinning process, the activities of the protein nanofibrous membranes were monitored. GOD remained active in the new membrane structure. The highest enzyme activity was observed for the membranes prepared with a 1.5:1 (v:v) TFE:PBS solvent ratio. In that particular case, the immobilized enzyme created a current of 0.7 mu A and the apparent activity was 2547 +/- 132 U/m(2). (C) 2016 Published by Elsevier B.V.en_US
dc.language.isoenen_US
dc.publisherElsevier Science Bven_US
dc.relation.ispartofReactive & Functional Polymersen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectbiocatalytic membraneen_US
dc.subjectamperometric detectionen_US
dc.subjectelectrospinningen_US
dc.subjectbovine serum albuminen_US
dc.subjectglucose oxidaseen_US
dc.titleBiocatalytic protein membranes fabricated by electrospinningen_US
dc.typeArticleen_US
dc.departmentFaculties, Faculty of Engineering, Department of Biomedical Engineeringen_US
dc.departmentFakülteler, Mühendislik Fakültesi, Biyomedikal Mühendisliği Bölümütr_TR
dc.identifier.volume103
dc.identifier.startpage26
dc.identifier.endpage32
dc.relation.tubitakTUBA-GEBIP awarden_US
dc.relation.tubitakScientific and Technological Research Council of Turkey (TUBITAK) [215Z047]en_US
dc.authorid0000-0002-8594-3459-
dc.authorid0000-0001-7146-1937-
dc.identifier.wosWOS:000377737800004en_US
dc.identifier.scopus2-s2.0-84964370985en_US
dc.institutionauthorMutlu, Mehmet-
dc.institutionauthorKabay, Gözde-
dc.identifier.doi10.1016/j.reactfunctpolym.2016.03.015-
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.identifier.scopusqualityQ1-
item.cerifentitytypePublications-
item.grantfulltextnone-
item.openairetypeArticle-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.fulltextNo Fulltext-
item.languageiso639-1en-
crisitem.author.dept02.2. Department of Biomedical Engineering-
Appears in Collections:Biyomedikal Mühendisliği Bölümü / Department of Biomedical Engineering
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection
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