Please use this identifier to cite or link to this item: https://hdl.handle.net/20.500.11851/1032
Title: Immobilization of superoxide dismutase/catalase onto polysulfone membranes to suppress hemodialysis-induced oxidative stress: A comparison of two immobilization methods
Authors: Mahlicli, Filiz Yaşar
Şen, Yasin
Mutlu, Mehmet
Altınkaya, Sacide Alsoy
Keywords: plasma treatment
self assembly of polyelectrolyte (ionic immobilization)
polysulfone membrane
catalase
superoxide dismutase
Issue Date: Apr-2015
Publisher: Elsevier Science Bv
Source: Mahlicli, F. Y., Şen, Y., Mutlu, M., & Altinkaya, S. A. (2015). Immobilization of superoxide dismutase/catalase onto polysulfone membranes to suppress hemodialysis-induced oxidative stress: A comparison of two immobilization methods. Journal of membrane science, 479, 175-189.
Abstract: The objective of this study is to improve the blood compatibility of polysullone (PSF) based hemodialysis membranes through generating antioxidative surfaces with superoxide dismutase (SOD)/catalase (CAT) enzyme couple immobilization. Enzymes were attached both covalently and ionically on the plasma treated and polyethyleneimine (PEI) deposited membranes, respectively. The loss of enzymes from PEI modified surface at the end of 4 h was found to be relatively higher during storage in phosphate buffered saline (PBS) at pH 74 when compared to the enzymes on the plasma treated surface. The kinetic studies indicated that SOD catalyzed the reaction in the diffusion-limited regime at all substrate concentrations and its inactivation by hydrogen peroxide was prevented in the presence of CAT. SOD/CAT coated PSF membranes were capable of reducing the levels of reactive oxygen species in blood and can significantly prolong activated partial thromboplastin time. In addition, both the adsorption of human plasma proteins and platelet activation on all modified membranes decreased significantly compared to the unmodified PSF membranes. Proposed modification methods did not affect high permeability, high mechanical strength or the non-toxic properties of the PSF membranes. (c) 2015 Elsevier B.V. All rights reserved.
URI: https://doi.org/10.1016/j.memsci.2014.12.025
https://hdl.handle.net/20.500.11851/1032
ISSN: 0376-7388
Appears in Collections:Biyomedikal Mühendisliği Bölümü / Department of Biomedical Engineering
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection

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