Please use this identifier to cite or link to this item: https://hdl.handle.net/20.500.11851/10707
Title: Circulating miRNAs and their functional genetic variants in pseudoexfoliative glaucoma: potential of miR-146a-5p as a diagnostic biomarker
Authors: Demirdoğen, Birsen Can
Özturk Başer, Tuğba
Köylü, Mehmet Talay
Özge, Gökhan
Köz, Özlem Gürbüz
Mumcuoğlu, Tarkan
Keywords: Biomarker
MicroRNA
Plasma
Risk
Susceptibility
Aqueous-Humor
Expression
Cancer
Micrornas
Identification
Inflammation
Polymorphism
Association
Deficiency
Eyes
Issue Date: 2023
Publisher: Springer
Abstract: PurposeThe etiology and pathogenesis of pseudoexfoliation syndrome (PEX) and its advancement into pseudoexfoliative glaucoma (PEG) are not fully understood. In this study, we aimed to evaluate the possible role played by two circulating microRNAs (miR-146a-5p and miR-196a-5p) in plasma and their functional genetic variants MIR146A rs2910164 and MIR196A2 rs11614913 in susceptibility to PEG or PEX.MethodsPlasma miRNA relative expression of 27 patients with PEG, 25 patients with PEX and 27 controls was determined using quantitative RT-PCR, and fold change was calculated using the 2(-& UDelta;& UDelta;Ct) method. Genotyping of 300 patients with PEG, 300 patients with PEX, and 300 controls was performed using a PCR-restriction fragment length polymorphism analysis.ResultPlasma miR-146a-5p relative expression was significantly elevated in patients with PEG (3.9-fold) (P < .000) and patients with PEX (2.7-fold) relative to controls (P = .001). The diagnostic ability of plasma miR-146a-5p expression fold change was good for discriminating PEG vs. controls (AUC = 0.897, P < .000), and the optimal decision threshold was 1.83 (sensitivity = 74%, specificity = 93%). Plasma miR-196a-5p relative expression did not differ significantly between study groups. No significant difference in terms of the minor allele frequency or the distribution of genotypes for MIR146A rs2910164 G/C or MIR196A2 rs11614913 C/T was observed between study groups.ConclusionsCirculating miR-146a-5p can contribute to the risk of PEX/PEG. Therefore, we propose that plasma miR-146a-5p can be developed as a potential biomarker for the minimally invasive diagnoses of PEX/PEG and as a potential therapeutic target with further studies.
URI: https://doi.org/10.1007/s10792-023-02797-w
https://hdl.handle.net/20.500.11851/10707
ISSN: 0165-5701
1573-2630
Appears in Collections:PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection

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