Demonstration of the Decrease in Locomotor Activity and Central Nervous System in the Demyelination Model, in Which the Toxic Agent is Realized by Gavage

Abstract

Introduction: The cuprizone model is a well-established instance to study demyelination and remyelination in rodents. The primary aim of this study was to demonstrate the loss of function in motor activity. Second, it was evaluated together with the changes in the amount of myelin in the ongoing process. Materials and Methods: This study is based on the administration of the cuprizone model in male C57BL/6 mice by oral gavage. The advantage of the oral gavage model is that mice were subjected to the equal dose of cuprizone. For this reason, the nonequal in demyelination was minimized. We have designed four groups, including demyelination/control and remyelination/control. Results: The results of the walking test and open field test showed that locomotor activity in the demyelination group deteriorated. Increased glial fibrillary acidic protein and decreased myelin basic protein expressions were shown in the corpus callosum of the demyelination group compared to the control and remyelination groups. The g-ratio of the demyelination group was calculated 0.86 ± 0.07, the g-ratio of the demyelination control group was calculated 0.66 ± 0.1, the g-ratio of the remyelination group was calculated 0.83 ± 0.06, and the g-ratio of the remyelination control group was calculated 0.76 ± 0.09. Discussion and Conclusion: In conclusion, in this demyelination model, which was applied differently from the literature, in our study, the behavioral effect on motor activity and to what extent it appeared histologically was evaluated. Thus, investigating the loss of function in motor activity as well as histological examination increased the reliability of the model we created in our study. © 2023 Journal of the Anatomical Society of India.