Please use this identifier to cite or link to this item: https://hdl.handle.net/20.500.11851/11044
Title: Investigation of the effects of Toxoplasma gondii on behavioral and molecular mechanism in bradyzoite stage
Authors: Akcay, G.
Bahadir, A.
Tatar, Y.
Nuri, Atalar, M.
Babur, C.
Taylan, Ozkan, A.
Keywords: Anxiety
Bradyzoite
Brain
Dopamine
Learning and Memory
Norepinephrine
Toxoplasma gondii
dihydroxyphenylacetic acid
dopamine
levodopa
noradrenalin
amygdala
animal behavior
animal experiment
animal model
animal tissue
anxiety
Article
bradyzoite
brain tissue
controlled study
corpus striatum
elevated plus maze test
hippocampal tissue
hippocampus
inoculation
learning
locomotion
male
memory
mouse
nonhuman
novel object recognition test
open field test
prefrontal cortex
Toxoplasma gondii
Issue Date: 2024
Publisher: Elsevier B.V.
Abstract: Toxoplasma gondii is a single-celled parasite that causes a disease called toxoplasmosis. It can reach the central nervous system, but the mechanism of T. gondii disrupting the functioning of these brain regions occurs in bradyzoite stage of parasite, causing brain damage by forming tissue cysts in brain. In our study, the effects of T. gondii on locomotor activity, anxiety, learning and memory, and norepinephrine (NE), levodopa (L-DOPA), dopamine (DA) and 3,4-D-dihydroxyphenylacetic acid (DOPAC) catecholamines in amygdala, striatum, prefrontal cortex and hippocampus regions of the brain were investigated in bradyzoite stage. Twenty male Albino mice Mus musculus, 4–5 weeks old, weighing 20–25 g, were used. T. gondii inoculated to mice intraperitonealy with 48–50-hour passages of T. gondii RH Ankara strain. For intraperitoneal inoculation of mice 5x104 tachyzoites per mouse. No inoculation was made in control group (n: 20). Locomotor activity behavior in open field test (OFT), anxious behavior in elevated plus maze (EPM), and learning behavior in novel object recognition (NOR) tests were evaluated. NE, L-DOPA, DA and DOPAC were measured by HPLC in brain tissues of amygdala, striatum, prefrontal cortex and hippocampus. A decrease was observed in the locomotor activity, anxiety and learning values of the T. gondii group compared to the control group (p < 0.05). The heighten in NE and L-DOPA levels in amygdala tissue of T. gondii group compared to control group, an elevation in NE, L-DOPA, DA and DOPAC levels in striatum tissue, and an increase in levels of NE in prefrontal cortex tissue were detected in monoamine results. In hippocampus tissue, an increase was observed in DA levels, while a decrease was observed in NE, L-DOPA and DOPAC levels. In our study, it has been shown that T. gondii in bradyzoite stage reduces locomotor activity, causes learning and memory impairment, and has anxiogenic effects. © 2024 Elsevier B.V.
URI: https://doi.org/10.1016/j.brainres.2024.148762
https://hdl.handle.net/20.500.11851/11044
ISSN: 0006-8993
Appears in Collections:PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection

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