Please use this identifier to cite or link to this item: https://hdl.handle.net/20.500.11851/11765
Title: Silica Nanoparticles Tailored with a Molecularly Imprinted Copolymer Layer as a Highly Selective Biorecognition Element
Authors: Oluz, Zehra
Yazlak, Mustafa Gokturk
Kursun, Tugana Talya
Nayab, Sana
Glasser, Günnar
Yameen, Basit
Duran, Hatice
Keywords: Boc-phenylalanine
molecularly imprinted polymers
optical biosensor
RAFT polymerization
silica nanoparticle
surface imprinting
surface plasmon resonance
Polymer-Films
Merit Enhancement
Amino-Acids
Surface
Recognition
Enantioseparation
Fluorescence
Nanospheres
Extraction
Sensors
Publisher: Wiley-V C H Verlag Gmbh
Abstract: Molecularly imprinted silica nanoparticles (SP-MIP) are synthesized for the real-time optical detection of low-molecular-weight compounds. Azo-initiator-modified silica beads are functionalized through reversible addition-fragmentation chain transfer (RAFT) polymerization, which leads to efficient control of the grafted layer. The copolymerization of methacrylic acid (MAA) and ethylene glycol dimethacrylate (EDMA) on azo initiator-coated silica particles (approximate to 100 nm) using chain transfer agent (2-phenylprop-2-yl-dithiobenzoate) is carried out in the presence of a target analyte molecule (l-Boc-phenylalanine anilide, l-BFA). The chemical and morphological properties of SP-MIP are characterized by scanning electron microscopy, X-ray photoelectron spectroscopy, Brunauer-Emmett-Teller surface analysis, and thermogravimetric analysis. Finally, SP-MIP is located on the gold surface to be used as a biorecognition layer on the surface plasmon resonance spectrometer (SPR). The sensitivity, response time, and selectivity of SP-MIP are investigated by three similar analogous molecules (l-Boc-Tryptophan, l-Boc-Tyrosine, and l-Boc-Phenylalanine) and the imprinted particle surface showed excellent relative selectivity toward l-Boc-Phenylalanine (l-BFA) (k = 61), while the sensitivity is recorded as limit of detection = 1.72 x 10(-4) m.
URI: https://doi.org/10.1002/marc.202400471
https://hdl.handle.net/20.500.11851/11765
ISSN: 1022-1336
1521-3927
Appears in Collections:PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection

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