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Title: Screening of Variations in CD22 Gene in Children with B-Precursor Acute Lymphoblastic Leukemia.
Authors: Öner, Deniz Aslar
Akın, D. F.
Sipahi, K.
Mumcuoğlu, M.
Ezer, U.
Kürekçi, A. E.
Akar, Mehmet Nejat
Keywords: Lymphocyte Antigen Receptor
Exon 12 Deletion
Signal-Transduction; Negative Regulator; Binding
Issue Date: Sep-2016
Publisher: Mary Ann Liebert, Inc.
Source: Aslar Öner, D., Akın, D. F., Sipahi, K., Mumcuoğlu, M., Ezer, U., Kürekçi, A. E., & Akar, N. (2016). Screening of variations in CD22 gene in children with B-precursor acute lymphoblastic leukemia. Genetic Testing and Molecular Biomarkers, 20(9), 552-555. doi:10.1089/gtmb.2016.0006
Abstract: Background: CD22 is expressed on the surface of B-cell lineage cells from the early progenitor stage of pro-B cell until terminal differentiation to mature B cells. It plays a role in signal transduction and as a regulator of B-cell receptor signaling in B-cell development. Objectives: We aimed to screen exons 9-14 of the CD22 gene, which is a mutational hot spot region in B-precursor acute lymphoblastic leukemia (pre-B ALL) patients, to find possible genetic variants that could play role in the pathogenesis of pre-B ALL in Turkish children. Methods: This study included 109 Turkish children with pre-B ALL who were diagnosed at Losante Hospital for Children with Leukemia. Genomic DNA was extracted from both peripheral blood and bone marrow leukocytes. Gene amplification was performed with PCR, and all samples were screened for the variants by single strand conformation polymorphism. Samples showing band shifts were sequenced on an automated sequencer. Results: In our patient group a total of 9 variants were identified in the CD22 gene by sequencing: a novel variant in intron 10 (T2199G); a missense variant in exon 12; 5 intronic variants between exon 12 and intron 13; a novel intronic variant (C2424T); and a synonymous in exon 13. Thirteen of 109 children (11.9%) carried the T2199G novel intronic variant located in intron 10, and 17 of 109 children (15.6%) carried the C2424T novel intronic variant. Conclusion: Novel variants in the CD22 gene in children with pre-B ALL in Turkey that are not present, in the Human Gene Mutation Database or NCBI SNP database, were found.
ISSN: 1945-0265
Appears in Collections:Dahili Tıp Bilimleri Bölümü / Department of Internal Medical Sciences
PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection

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