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Title: Protective effect of resveratrol in di-n-butyl phthalate-induced nephrotoxicity: Immunohistochemical and ultrastructural studies
Authors: Elmas, Çiğdem
Seymen, Cemile Merve
Şener, Dila
Göktaş, Güleser
Göktaş, Tayfun
Türkkanı, Ayten
Keywords: Butyl phthalate
di-n-butyl phthalate
ET1 protein
Scanning transmission electron microscopy tomography
TEM tomography
Transmission electron microscopy
Issue Date: Jan-2017
Publisher: Science Printers and Publishers, Inc.
Source: Elmas, C., Seymen, C. M., Sener, D., Göktas, G., Göktas, T., & Türkkanı, A. (2017). Protective Effect of Resveratrol in Di–n-butyl Phthalate–Induced Nephrotoxicity.
Abstract: Objective: To explore the renoprotective nature of resveratrol by assessing markers of antioxidant competence in di–n-butyl phthalate (DBP)–injured rat kidneys with immunohistochemistry and electron microscopic techniques and as well as biochemical analyses. Study Design: A total of 36 adult female 20-day-old Wistar albino rats were given a diet containing either 500 mg/kg/day DBP (low-dose group) or 1,000 mg/kg/day DBP (high-dose group) dissolved in corn oil for 4 weeks. To study the potential protective effects of resveratrol and the effects of a solvent for resveratrol, other groups were used as controls and were given a solvent (carboxymethyl cellulose [CMC], 10 mL/kg), 500 mg/kg/day DBP+20 mg/kg/day resveratrol, or 1,000 mg/kg/day DBP+20 mg/kg/day resveratrol. Results: DBP and CMC treatment increased renal lipid peroxidation significantly and decreased the RSH level. TEM and SEM results showed degenerative changes such as deletion, folding and thickening of basement membrane, appearance of electron-dense intramembranous and mesangial deposits, and deletion of foot processes in the high-dose DBP-treated group. Treatment with resveratrol led to an improvement in both biochemical and histological alterations induced by DBP or CMC. Immunohistochemical results also supported our electron microscopic findings. Conclusion: DBP caused renal toxicity by inducing lipid peroxidation and morphological alterations, and resveratrol protects against DBP-induced nephro- toxicity.
ISSN: 0301-102X
Appears in Collections:Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
Temel Tıp Bilimleri Bölümü / Department of Basic Medical Sciences
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection

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