Please use this identifier to cite or link to this item: https://hdl.handle.net/20.500.11851/4005
Title: Melatonin attenuates ovarian ischemia reperfusion injury in rats by decreasing oxidative stress index and peroxynitrite
Authors: Kalyoncu, Şenol
Yılmaz, Bülent
Demir, Mustafa
Tuncer, Meltem
Bozdağ, Zehra
İnce, Onur
Bozdayı, Mehmet Akif
Ulusal, Hasan
Taysı, Seyithan
Keywords: Ischemia reperfusion injury
rat ovarian torsion
melatonin
oxidative stress index
peroxynitrite
Issue Date: 2020
Publisher: TÜBİTAK
Source: Kalyoncu, Ş., Yilmaz, B., Demir, M., Tuncer, M., Bozdağ, Z., Ince, O., ... & Taysi, S. (2020). Melatonin attenuates ovarian ischemia reperfusion injury in rats by decreasing oxidative stress index and peroxynitrite. Turkish Journal of Medical Sciences, 50(6), 1513-1522.
Abstract: Background/aim: To evaluate the protective effect of melatonin on ovarian ischemia reperfusion injury in a rat model. Materials and methods: Forty-eight rats were separated equally into 6 groups. Group 1: sham; Group 2: surgical control with 3-h bilateral ovarian torsion and detorsion; Group 3: intraperitoneal 5% ethanol (1 mL) just after detorsion (as melatonin was dissolved in ethanol); Group 4: 10 mg/kg intraperitoneal melatonin 30 min before 3-h torsion; Group 5:10 mg/kg intraperitoneal melatonin just after detorsion; Group 6:10 mg/kg intraperitoneal melatonin 30 min before torsion and just after detorsion. Both ovaries and blood samples were obtained 7 days after detorsion for histopathological and biochemical analysis. Results: In Group 1, serum levels of total oxidant status (TOS) (?mol H2 O2 equivalent/g wet tissue)were significantly lower than in Group2 (P = 0.0023), while tissue TOS levels were lower than in Group 3 (P = 0.0030). Similarly, serum and tissue levels of peroxynitrite in Group 6were significantly lower than those ofGroup 2 (P = 0.0023 and P = 0.040, respectively). Moreover, serum oxidative stress index (OSI) (arbitrary unit) levels were significantly increased in Group 2 when compared to groups 1 and 6 (P = 0.0023 and P= 0.0016, respectively) and in Group 3 with respect to groups 1, 4, 5, and 6 (P = 0.0023, P = 0.0026, P = 0.0008, and P = 0.0011, respectively). Furthermore, there was a significant decrease in histopathological scores including follicular degeneration, vascular congestion, hemorrhage, and inflammation in the melatonin and sham groups in comparison with control groups. Additionally, primordial follicle count was significantly higher in Group 6 than in Group 2 (P = 0.0002). Conclusion: Melatonin attenuates ischemia reperfusion damage in a rat torsion/detorsion model by improving histopathological and biochemical findings including OSI and peroxynitrite.
URI: https://hdl.handle.net/20.500.11851/4005
https://journals.tubitak.gov.tr/medical/abstract.htm?id=28043
ISSN: 1300-0144
Appears in Collections:Cerrahi Tıp Bilimleri Bölümü / Department of Surgical Sciences
PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection

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