Please use this identifier to cite or link to this item: https://hdl.handle.net/20.500.11851/5522
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dc.contributor.authorZırh, S.-
dc.contributor.authorErol, S.-
dc.contributor.authorZırh, E. B.-
dc.contributor.authorSökmensuer, L. K.-
dc.contributor.authorBozdağ, G.-
dc.contributor.authorMüftüoğlu, S. F.-
dc.date.accessioned2021-09-11T15:19:10Z-
dc.date.available2021-09-11T15:19:10Z-
dc.date.issued2021en_US
dc.identifier.issn1389-9333-
dc.identifier.urihttps://doi.org/10.1007/s10561-021-09929-5-
dc.identifier.urihttps://hdl.handle.net/20.500.11851/5522-
dc.description.abstractGranulosa cells are the cell population who have an increasing importance in the female genital system and reproduction. Thus, nowadays in vitro studies to address these cells are also gaining importance and attracts researcher’s attention. The aim of our study is to develop a more feasible, low-cost granulosa cell isolation and culture method compared to methods defined so far. Granulosa cells were isolated from follicular fluids obtained from both healthy women donors (n = 19) and polycystic ovary syndrome (n = 15) applied to in vitro fertilization treatment process. Granulosa cells were isolated by using Lymphosep® separation fluid that was not used for this purpose before. The isolated cells were cultured in suitable culture dishes with a mixture of BIO-AMFTM-1 and DMEM/F12 in the first seeding and only complete DMEM/F12 in the following feeds. Complete medium contains only 5% fetal calf serum, 4% L-glutamine and 1% penicillin–streptomycin-amphotericin. The new methods we have developed in granulosa cell isolation and in vitro culture have been successful. Reduction in supplement types and amount; improved the proliferation rate of the granulosa cells in culture. Our new methods of isolation and cell culture for granulosa cells from healthy women, have been also successful in samples of polycystic ovarian patients. With these developed methods, granulosa cells, which belong to humans and have an important role in the ovary, could be isolated and subsequently be maintained to reproduce (proliferate) more easily and cheaper. © 2021, The Author(s), under exclusive licence to Springer Nature B.V.en_US
dc.description.sponsorshipTSA-2018-17320en_US
dc.language.isoenen_US
dc.publisherSpringer Science and Business Media B.V.en_US
dc.relation.ispartofCell and Tissue Bankingen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectCell cultureen_US
dc.subjectCell isolationen_US
dc.subjectGranulosa cellen_US
dc.subjectPolycystic ovaryen_US
dc.titleA new isolation and culture method for granulosa cellsen_US
dc.typeArticleen_US
dc.departmentFaculties, School of Medicine, Department of Basic Medical Sciencesen_US
dc.departmentFakülteler, Tıp Fakültesi, Temel Tıp Bilimleri Bölümütr_TR
dc.identifier.scopus2-s2.0-85105462970en_US
dc.institutionauthorBahador Zırh, Elham-
dc.identifier.pmid33914204en_US
dc.identifier.doi10.1007/s10561-021-09929-5-
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.identifier.scopusqualityQ3-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.grantfulltextnone-
item.fulltextNo Fulltext-
item.openairetypeArticle-
item.cerifentitytypePublications-
item.languageiso639-1en-
Appears in Collections:PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
Temel Tıp Bilimleri Bölümü / Department of Basic Medical Sciences
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