Please use this identifier to cite or link to this item: https://hdl.handle.net/20.500.11851/719
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dc.contributor.authorAltuntaş, Sevde-
dc.contributor.authorBüyükserin, Fatih-
dc.date.accessioned2019-03-15T13:55:06Z
dc.date.available2019-03-15T13:55:06Z
dc.date.issued2018-08-08
dc.identifier.citationAltuntas, S., & Buyukserin, F. (2018). Fabrication of thioflavin‐T‐modified nanopillared SERS substrates for ultrasensitive beta‐amyloid peptide detection. Journal of Raman Spectroscopy, 49(8), 1247-1256.en_US
dc.identifier.issn0377-0486
dc.identifier.urihttps://doi.org/10.1002/jrs.5376-
dc.identifier.urihttps://hdl.handle.net/20.500.11851/719-
dc.description.abstractAlzheimer disease (AD) is a major cause of death in the world, and despite intense efforts, there is still no cure for this neurodegenerative disorder. Therefore, early diagnosis of AD is of prime importance, and extensive studies are being conducted to improve the current early detection capabilities and to identify new potential biomarkers for this disease. Here in, we report the fabrication of a novel nanobiosensor platform for the surface-enhanced Raman spectroscopy (SERS)-based ultrasentisitive detection of beta-amyloid (1–42) peptide (Aβ[1–42]), a well-established biomarker of AD. Polymeric films having multibranched nanopillared surfaces (MNS) were first fabricated by drop-casting polycarbonate solutions onto anodized aluminum oxide molds that possess hierarchically branched pores. After peeling from the nanoporous molds, a 20-nm Au coating allowed these MNS substrates to sense sub-picomolar levels of Thioflavin-T (ThT), a SERS-active dye, clinically used to diagnose amyloid plaque presence. Of particular interest, the ThT-modified MNS films demonstrated a gradual ThT SERS signal suppression when incubated with increasing concentrations of Aβ(1–42), and this trend was used to obtain a limit of detection value of 0.5 pg/ml and a linear dynamic range between 0.5 pg/ml and 100 ng/ml. The developed biosensor platform has stable ThT SERS signals for extended periods and displayed promising results in artificial saliva. Ongoing studies are focused on its potential with real body fluids.en_US
dc.description.sponsorshipNational Council for Scientific Research[214Z167]en_US
dc.language.isoenen_US
dc.publisherJohn Wiley and Sons Ltden_US
dc.relation.ispartofJournal of Raman Spectroscopyen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectAβ(1‐42) detectionen_US
dc.subjectNanopillared filmsen_US
dc.subjectNanobiosensoren_US
dc.subjectSERSen_US
dc.subjectThTen_US
dc.titleFabrication of thioflavin-T-modified nanopillared SERS substrates for ultrasensitive beta-amyloid peptide detectionen_US
dc.typeArticleen_US
dc.departmentFaculties, Faculty of Engineering, Department of Biomedical Engineeringen_US
dc.departmentFakülteler, Mühendislik Fakültesi, Biyomedikal Mühendisliği Bölümütr_TR
dc.identifier.volume49
dc.identifier.issue8
dc.identifier.startpage1247
dc.identifier.endpage1256
dc.authorid0000-0001-6365-3808-
dc.identifier.wosWOS:000441696300001en_US
dc.identifier.scopus2-s2.0-85047622442en_US
dc.institutionauthorBüyükserin, Fatih-
dc.identifier.doi10.1002/jrs.5376-
dc.identifier.doi10.1002/jrs.5376-
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.identifier.scopusqualityQ2-
item.grantfulltextnone-
item.fulltextNo Fulltext-
item.languageiso639-1en-
item.cerifentitytypePublications-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.openairetypeArticle-
crisitem.author.dept02.2. Department of Biomedical Engineering-
Appears in Collections:Biyomedikal Mühendisliği Bölümü / Department of Biomedical Engineering
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection
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