Please use this identifier to cite or link to this item: https://hdl.handle.net/20.500.11851/7292
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dc.contributor.authorBaştuğ, Turgut-
dc.contributor.authorHeinzelmann, Germano-
dc.contributor.authorKuyucak, Serdar-
dc.contributor.authorSalim, Marietta-
dc.contributor.authorVandenberg, Robert J.-
dc.contributor.authorRyan, Renae M.-
dc.date.accessioned2021-09-11T15:56:17Z-
dc.date.available2021-09-11T15:56:17Z-
dc.date.issued2012en_US
dc.identifier.issn1932-6203-
dc.identifier.urihttps://doi.org/10.1371/journal.pone.0033058-
dc.identifier.urihttps://hdl.handle.net/20.500.11851/7292-
dc.description.abstractGlutamate transport via the human excitatory amino acid transporters is coupled to the co-transport of three Na+ ions, one H+ and the counter-transport of one K+ ion. Transport by an archaeal homologue of the human glutamate transporters, Glt(Ph), whose three dimensional structure is known is also coupled to three Na+ ions but only two Na+ ion binding sites have been observed in the crystal structure of GltPh. In order to fully utilize the Glt(Ph) structure in functional studies of the human glutamate transporters, it is essential to understand the transport mechanism of Glt(Ph) and accurately determine the number and location of Na+ ions coupled to transport. Several sites have been proposed for the binding of a third Na+ ion from electrostatic calculations and molecular dynamics simulations. In this study, we have performed detailed free energy simulations for GltPh and reveal a new site for the third Na+ ion involving the side chains of Threonine 92, Serine 93, Asparagine 310, Aspartate 312, and the backbone of Tyrosine 89. We have also studied the transport properties of alanine mutants of the coordinating residues Threonine 92 and Serine 93 in Glt(Ph), and the corresponding residues in a human glutamate transporter, EAAT1. The mutant transporters have reduced affinity for Na+ compared to their wild type counterparts. These results confirm that Threonine 92 and Serine 93 are involved in the coordination of the third Na+ ion in Glt(Ph) and EAAT1.en_US
dc.description.sponsorshipAustralian Research CouncilAustralian Research Council [DP1092729]; Turkish Scientific and Technical Research CouncilTurkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK); National Health and Medical Research CounciNational Health and Medical Research Council of Australia [571093]en_US
dc.description.sponsorshipThis work was supported by grants from the Australian Research Council (#DP1092729) and the Turkish Scientific and Technical Research Council. RMR is supported by a National Health and Medical Research Council Career Development Fellowship (#571093). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.en_US
dc.language.isoenen_US
dc.publisherPublic Library Scienceen_US
dc.relation.ispartofPlos Oneen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subject[No Keywords]en_US
dc.titlePosition of the Third Na+ Site in the Aspartate Transporter Glt(Ph) and the Human Glutamate Transporter, EAAT1en_US
dc.typeArticleen_US
dc.departmentFaculties, Faculty of Engineering, Department of Material Science and Nanotechnology Engineeringen_US
dc.departmentFakülteler, Mühendislik Fakültesi, Malzeme Bilimi ve Nanoteknoloji Mühendisliği Bölümütr_TR
dc.identifier.volume7en_US
dc.identifier.issue3en_US
dc.authorid0000-0003-1523-4814-
dc.authorid0000-0001-7168-6941-
dc.authorid0000-0001-9123-9292-
dc.authorid0000-0002-8680-4610-
dc.authorid0000-0003-4315-9532-
dc.identifier.wosWOS:000303129700029en_US
dc.identifier.scopus2-s2.0-84858110981en_US
dc.institutionauthorBaştuğ, Turgut-
dc.identifier.pmid22427946en_US
dc.identifier.doi10.1371/journal.pone.0033058-
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.identifier.scopusqualityQ1-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.grantfulltextnone-
item.fulltextNo Fulltext-
item.openairetypeArticle-
item.cerifentitytypePublications-
item.languageiso639-1en-
crisitem.author.dept02.6. Department of Material Science and Nanotechnology Engineering-
Appears in Collections:Malzeme Bilimi ve Nanoteknoloji Mühendisliği Bölümü / Department of Material Science & Nanotechnology Engineering
PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection
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