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Title: Biocatalytic protein membranes fabricated by electrospinning
Authors: Kabay, Gözde
Kaleli, Gizem
Sultanova, Zahida
Ölmez, Tolga Tarkan
Şeker, Urartu Özgür Şafak
Mutlu, Mehmet
Keywords: biocatalytic membrane
amperometric detection
bovine serum albumin
glucose oxidase
Issue Date: Jun-2016
Publisher: Elsevier Science Bv
Source: Kabay, G., Kaleli, G., Sultanova, Z., Ölmez, T. T., Şeker, U. Ö. Ş., & Mutlu, M. (2016). Biocatalytic protein membranes fabricated by electrospinning. Reactive and Functional Polymers, 103, 26-32.
Abstract: In this study, a protein-based catalytic membrane was produced by electrospinning. Membrane activity was characterised in terms of response current for various glucose concentrations. We focused on the preparation of a scaffold by converting a globular protein to other structural forms using catastrophic solvents. A scaffolding protein, bovine serum albumin, and an enzyme, glucose oxidase (GOD), were selected as a model natural carrier matrix and a biologically active agent, respectively. Beta-mercaptoethanol (beta-ME) was used to convert the globular protein to an amyloid-like form. A structural stabilising agent, 2,2,2-triflouroethanol (TFE), was used to maintain the final alpha-helical structure of the amyloid-like protein. The TFE:PBS (phosphate-buffered saline) ratio and various electrospinning parameters were analysed to minimise activity loss. Using this approach, we applied electrospinning to an active enzyme to obtain biocatalytic nanofibrous membranes. After optimising the protein electrospinning process, the activities of the protein nanofibrous membranes were monitored. GOD remained active in the new membrane structure. The highest enzyme activity was observed for the membranes prepared with a 1.5:1 (v:v) TFE:PBS solvent ratio. In that particular case, the immobilized enzyme created a current of 0.7 mu A and the apparent activity was 2547 +/- 132 U/m(2). (C) 2016 Published by Elsevier B.V.
ISSN: 1381-5148
Appears in Collections:Biyomedikal Mühendisliği Bölümü / Department of Biomedical Engineering
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection

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