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Title: Mixed monolayer decorated SPR sensing surface for thrombin detection
Authors: Sadık, Demet Ataman
Boyacı, İsmail Hakkı
Mutlu, Mehmet
Keywords: Biosensor
Gold surfaces
3,3 ' Dithiodipropionic acid di (N-hydroxysuccinimide ester)
DSP:6-mercapto-1-hexanol (MCH)
Mixed self-assembled monolayers (mSAMs)
Biological recognition element immobilization
Surface plasmon resonance (SPR)
Issue Date: 30-Oct-2019
Publisher: Elsevier B.V.
Source: Sadık, D. A., Boyacı, İ. H., & Mutlu, M. (2019). Mixed monolayer decorated SPR sensing surface for thrombin detection. Journal of pharmaceutical and biomedical analysis, 176, 112822.
Abstract: The development of surface plasmon resonance (SPR) based immunosensor for thrombin detection was aimed. For this purpose, 3,3? Dithiodipropionic acid di (N-hydroxysuccinimide ester) (DSP):6-mercapto-1-hexanol (MCH) mixed self-assembled monolayers (mSAMs) were formed on gold surfaces for immobilization of anti-thrombin antibody. The performance of the immunosensor was determined against the target protein thrombin at various concentrations using flow cell coupled SPR. The linear detection range of the immunosensor was 30.0–100.0 nM with an R2 value of 0,992. Limit of Detection (LOD) and Limit of Quantification (LOQ) were determined to be 6.0 nM and 30.0 nM, respectively. The selectivity of the immunosensor was tested against a non-target model protein, human serum albumin (HSA) and the obtained ?RU value was found to be below the ?RU value corresponding to the LOQ concentration for thrombin. The immunosensor's capability to detect thrombin in diluted complex serum matrix was also tested and the obtained ?RU value (159 ± 16) was compared with ?RU value obtained for thrombin detection in PBS solution (137 ± 19). Based on the results, it was shown that DSP:MCH interface is a promising immobilization platform for binding biological recognition elements for the development of biosensors.
ISSN: 0731-7085
Appears in Collections:Biyomedikal Mühendisliği Bölümü / Department of Biomedical Engineering
PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection

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