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Title: Medically important Candida spp. identification: an era beyond traditional methods
Authors: Evren E.
Göçmen J.S.
Hortaç Istar E.
Yavuzdemir S.
Tekeli A.
Yavuz Y.
Karahan Z.C.
Keywords: Candida identification
CHROMagar Candida
corn meal tween-80 agar
antifungal susceptibility
Candida albicans
Candida glabrata
Candida parapsilosis
Candida tropicalis
controlled study
DNA sequencing
fungus identification
mass spectrometry
matrix assisted laser desorption ionization time of flight mass spectrometry
polymerase chain reaction
quality control
sequence analysis
Issue Date: 2022
Publisher: Turkiye Klinikleri
Abstract: Background/aim: Candida infections are gaining more attention for the last few decades so diagnostic tools are very important for early diagnosis. Conventional identification of yeasts is time-consuming, molecular methods are more complicated and relatively expensive gold-standard methods. Matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS) was put into the market due to its speed and high accuracy. The aim of this study was to evaluate the performance of corn meal tween-80 agar (CMTA), CHROMagar Candida medium, and MALDI-TOF MS and to compare the obtained results with DNA sequencing. Materials and methods: The CHROMagar Candida medium, CMTA, and MALDI-TOF MS Biotyper System were used to test 416 isolates. The isolates with discrepant results by at least one of the three methods were subjected to sequence analysis. Results: The identification results of the 351 (%84.4) were compatible with all three methods. When compared to the sequencing results, the most accurate results were obtained by the MALDI-TOF MS, especially for rare Candida species. Conclusion: MALDI-TOF MS is found to be the most accurate identification tool for clinically important Candida strains. CMTA alone should not be used for the final identification of Candida species and the chromogenic medium should always be considered presumptive. © TÜBİTAK.
ISSN: 1300-0144
Appears in Collections:Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
Temel Tıp Bilimleri Bölümü / Department of Basic Medical Sciences
TR Dizin İndeksli Yayınlar / TR Dizin Indexed Publications Collection

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