Please use this identifier to cite or link to this item: https://hdl.handle.net/20.500.11851/9402
Title: The Effects of Transcranial Focused Ultrasound Stimulation of Nucleus Accumbens on Neuronal Gene Expression and Brain Tissue in High Alcohol-Preferring Rats
Authors: Deveci, Erdem
Akbas, Fahri
Ergün, Arif Şanlı
Kurtulmuş, Ayse
Koçak, Ali Barlas
Boyraz, Rabia Kevser
Kırpınar İsmet
Keywords: Low-intensity focused ultrasound (LIFU)
Alcohol dependency
Nucleus accumbens
miRNA
Neuromodulation
Frontal-Cortex
Microrna
Ethanol
Addiction
Cells
Neuromodulation
Dysregulation
Activation
History
Disease
Issue Date: 2022
Publisher: Springer
Abstract: We investigated the effect of low-intensity focused ultrasound (LIFU) on gene expression related to alcohol dependence and histological effects on brain tissue. We also aimed at determining the miRNA-mRNA relationship and their pathways in alcohol dependence-induced expression changes after focused ultrasound therapy. We designed a case-control study for 100 days of observation to investigate differences in gene expression in the short-term stimulation group (STS) and long-term stimulation group (LTS) compared with the control sham group (SG). The study was performed in our Experimental Research Laboratory. 24 male high alcohol-preferring rats 63 to 79 days old, weighing 270 to 300 g, were included in the experiment. LTS received 50-day LIFU and STS received 10-day LIFU and 40-day sham stimulation, while the SG received 50-day sham stimulation. In miRNA expression analysis, it was found that LIFU caused gene expression differences in NAc. Significant differences were found between the groups for gene expression. Compared to the SG, the expression of 454 genes in the NAc region was changed in the STS while the expression of 382 genes was changed in the LTS. In the LTS, the expression of 32 genes was changed in total compared to STS. Our data suggest that LIFU targeted on NAc may assist in the treatment of alcohol dependence, especially in the long term possibly through altering gene expression. Our immunohistochemical studies verified that LIFU does not cause any tissue damage. These findings may lead to new studies in investigating the efficacy of LIFU for the treatment of alcohol dependence and also for other psychiatric disorders.
URI: https://doi.org/10.1007/s12035-022-03130-9
https://hdl.handle.net/20.500.11851/9402
ISSN: 0893-7648
1559-1182
Appears in Collections:PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection

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